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KMID : 0352720140380030208
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Journal of Ginseng Research
2014 Volume.38 No. 3 p.208 ~ p.214
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Korean Red Ginseng saponin fraction modulates radiation effects on lipopolysaccharide-stimulated nitric oxide production in RAW264.7 macrophage cells
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Lee Young-Ji
Han Jeong-Yoon
Lee Chang-Geun
Heo Kyu
Park Se-Il
Park Yoo-Soo
Kim Joong-Sun
Yang Kwang-Mo
Lee Ki-Ja
Kim Tae-Hwan
Rhee Man-Hee
Kim Sung-Dae
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Abstract
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Background: In previous work, we reported that Korean Red Ginseng saponin fraction (RGSF) showed anti-inflammatory activities in vitro and in vivo. Methods: The present study investigated the radioprotective properties of RGSF by examining its effects on ionizing radiation (IR)-enhanced and lipopolysaccharide (LPS)-mediated inflammatory responses in murine macrophage cells. Results: RGSF induced strong downregulation of IR-enhanced and LPS-induced proinflammatory responses such as nitric oxide (NO) production (Inhibitory Concentration 50(IC50=5.1±0.8μM) and interleukin-1β levels. RGSF was found to exert its radioprotective effects by inhibition of a signaling cascade that activated checkpoint kinase 2enuclear factor-KB. In addition, RGSF strongly inhibited IR-enhanced LPS-induced expression of hemoxyganase-1, implying that the latter may be a potential target of RGSF. Conclusion: Taken together, our data suggest that RGSF can be considered and developed for use as an effective radioprotective agent with minimal adverse effects.
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KEYWORD
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20(S)-protopanaxadiol saponins, ionizing radiation, macrophages, Panax ginseng
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